| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | BCL2;Apoptosis regulator Bcl-2 |
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| Isotype | |
| Reactivity | |
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Product Overview
U-Blot® Bcl-2 (Phospho Ser70) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Bcl-2. Recognizes Phospho (Ser70). It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human.
Validated Applications
| Target | Bcl-2 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1385R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Bcl-2 (Phospho Ser70) Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at S70.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RTsPL |
| Molecular Weight | Calculated: 26 kDa | Observed: 26 kDa |
| UniProt / Swiss-Prot | P10415 (Human) | P10417 (Mouse) | P49950 (Rat) |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Apoptosis regulator Bcl-2
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1385R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
