U-Blot® β-1,4-GalNAc-T Rabbit Polyclonal Antibody

SKU:BHA21809961
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-β-1/4-GalNAc-T antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP15111-50 50 uL
WP15111-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target β-1/4-GalNAc-T
Antigen
  • Chondroitin sulfate N-acetylgalactosaminyltransferase 1
Alternative names CSGALNACT1;CHGN;GALNACT1;Chondroitin sulfate N-acetylgalactosaminyltransferase 1;CsGalNAcT-1;Chondroitin beta-1;4-N-acetylgalactosaminyltransferase 1;Beta4GalNAcT-1
Gene ID 55790 (Human) | 234356 (Mouse)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human CSGALNACT1. AA range:201-250
Conjugate
  • Unconjugated
Molecular weight Observed: 60 kDa
Purification Affinity Purified
Cellular localization Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP15111
Main SKU BHA21809961
Antibody

Product Overview

U-Blot® β-1,4-GalNAc-T Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against β-1/4-GalNAc-T. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target β-1/4-GalNAc-T
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity β-1,4-GalNAc-T Polyclonal Antibody detects endogenous levels of β-1,4-GalNAc-T protein.
Molecular Weight Observed: 60 kDa
UniProt / Swiss-Prot Q8TDX6 (Human) | Q8BJQ9 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human CSGALNACT1. AA range:201-250

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronyl-(1->3)-D-galactosyl-proteoglycan = UDP + N-acetyl-D-galactosaminyl-(1->4)-beta-D-glucuronyl-(1->3)-beta-D-galactosylproteoglycan.,function:Transfers 1,4-N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of glucuronic acid (GlcUA). Required for addition of the first GalNAc to the core tetrasaccharide linker and for elongation of chondroitin chains. Important role in chondroitin chain biosynthesis in cartilage.,online information:Chondroitin beta-1,4-N-acetylgalactosaminyltransferase 1,online information:GlycoGene database,PTM:N-glycosylated.,similarity:Belongs to the chondroitin N-acetylgalactosaminyltransferase family.,tissue specificity:Ubiquitous, with the highest levels in placenta, thyroid, bladder, prostate and adrenal gland. Detected at low levels in the other tissues examined.

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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