U-Blot® β-actin Mouse Monoclonal Antibody (5B7), Cy3 Conjugated

SKU:BHA21802513
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Actin β antibody (Mouse host, monoclonal, Cy3). Validated for Immunofluorescence, Western Blot, Immunohistochemistry with reactivity to Human, Rat, Mouse, Monkey.
Clonality Monoclonal
Host Mouse
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect
Conjugate Cy3
Isotype IgG1
Clone 5B7
Applications Immunofluorescence, Western Blot, Immunohistochemistry
Options selector
Catalog no. Size
WM0948-50 50 uL
WM0948-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Actin β
Antigen
  • Actin cytoplasmic 1
Alternative names ACTB;Actin;cytoplasmic 1;Beta-actin;Actin β;β-Actin;Beta actin;B actin;actin Beta
Gene ID 60 (Human)
Host Mouse
Clone 5B7
Clonality
  • Monoclonal
Isotype
  • IgG1
Reactivity
  • Human
  • Rat
  • Mouse
  • Mk
  • Dg
  • Ch
  • Hamster
  • Rabbit
  • Insect
Applications
  • Immunofluorescence
  • Western Blot
  • Immunohistochemistry
Conjugate
  • Cy3
Purification Affinity Purified
Cellular localization Cytoplasm, cytoskeleton . Nucleus . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Concentration 1 mg/ml
Formulation Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Storage Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Catalog no. (Mfr.) WM0948
Main SKU BHA21802513
Antibody

Product Overview

U-Blot® β-actin Mouse Monoclonal Antibody (5B7), Cy3 Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Actin β. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry and reacts with Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect.

Validated Applications

Target Actin β
Host Mouse
Clonality Monoclonal
Clone 5B7
Isotype IgG1
Conjugate Cy3
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect
Applications Immunofluorescence, Western Blot, Immunohistochemistry
Purification Affinity Purified
Specificity β-actin Monoclonal Antibody(5B7) Cy3 Conjugated specially designed for your Immunofluorescence analysis.
UniProt / Swiss-Prot P60709 (Human)

Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:WB 1:5000;IHC 1:200.

Immunogen / Antigen

Actin cytoplasmic 1

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect. Confirm suitability for untested species before use.

Scientific Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
What applications is this antibody validated for?

This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:WB 1:5000;IHC 1:200.

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Cy3.

How should it be stored?

Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.

What is the clone number?

Clone 5B7.

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