| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CTNNB1;CTNNB;OK/SW-cl.35;Catenin beta-1;Beta-catenin |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
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Product Overview
U-Blot® β Catenin (Phospho Ser552) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Catenin-β Phospho Ser552. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Catenin-β Phospho Ser552 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1629R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | β Catenin (Phospho Ser552) Antibody detects endogenous levels of Catenin-β protein only when phosphorylated at S552.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RTsMG |
| Molecular Weight | Calculated: 85 kDa | Observed: 100 kDa |
| UniProt / Swiss-Prot | P35222 (Human) | Q02248 (Mouse) | Q9WU82 (Rat) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Catenin-β;b-catenin;Beta catenin;Beta-catenin;Cadherin associated protein;Catenin (cadherin associated protein), beta 1, 88 kDa;Catenin beta 1;Catenin beta-1;CATNB;CHBCAT;CTNB1_HUMAN;CTNNB;CTNNB1;DKFZp686D02253;FLJ25606;FLJ37923;OTTHUMP00000162082;OTTHUMP00000165222;OTTHUMP00000165223;OTTHUMP00000209288;OTTHUMP00000209289
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1629R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
