U-Blot® β-Tubulin Mouse Monoclonal Antibody (5G3), AbFluor 555 Conjugated

SKU:BHA21802519
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Tubulin β antibody (Mouse host, monoclonal, AbFluor 555). Validated for Western Blot, Immunofluorescence, Immunohistochemistry with reactivity to Human, Rat, Mouse, Monkey.
Clonality Monoclonal
Host Mouse
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast
Conjugate AbFluor 555
Isotype IgG1
Clone 5G3
Applications Western Blot, Immunofluorescence, Immunohistochemistry
Options selector
Catalog no. Size
WM0954-50 50 uL
WM0954-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Tubulin β
Antigen
  • Tubulin beta-3 chain
Alternative names TUBB3
Gene ID 10381 (Human)
Host Mouse
Clone 5G3
Clonality
  • Monoclonal
Isotype
  • IgG1
Reactivity
  • Human
  • Rat
  • Mouse
  • Mk
  • Dg
  • Ch
  • Hamster
  • Rabbit
  • sheep
  • Insect
  • Yeast
Applications
  • Western Blot
  • Immunofluorescence
  • Immunohistochemistry
Conjugate
  • AbFluor 555
Purification Affinity Purified
Cellular localization Cytoplasm, cytoskeleton . Cell projection, growth cone . Cell projection, lamellipodium . Cell projection, filopodium .
Concentration 1 mg/ml
Formulation Liquid in PBS, pH 7.4, containing 0.02% sodium azide as preservative and 50% Glycerol.
Storage Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
Catalog no. (Mfr.) WM0954
Main SKU BHA21802519
Antibody

Product Overview

U-Blot® β-Tubulin Mouse Monoclonal Antibody (5G3), AbFluor 555 Conjugated is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Tubulin β. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry and reacts with Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast.

Validated Applications

Target Tubulin β
Host Mouse
Clonality Monoclonal
Clone 5G3
Isotype IgG1
Conjugate AbFluor 555
Reactivity Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast
Applications Western Blot, Immunofluorescence, Immunohistochemistry
Purification Affinity Purified
Specificity β-Tubulin Monoclonal Antibody(5G3) AbFluor™ 555 Conjugated specially designed for your Immunofluorescence analysis.
UniProt / Swiss-Prot Q13509 (Human)

Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.

Immunogen / Antigen

Tubulin beta-3 chain

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast. Confirm suitability for untested species before use.

Scientific Background

tubulin beta 3 class III(TUBB3) Homo sapiens This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry. Recommended dilutions: Optimal working dilutions should be determined experimentally by the investigator;Suggested starting dilutions are as follows:IHC 1:200;IF 1:200.

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Sheep, Insect, Yeast. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: AbFluor 555.

How should it be stored?

Store as follows: Stable for one year at -15°C to -25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Store in dark.

What is the clone number?

Clone 5G3.

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