| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ARNTL;BHLHE5;BMAL1;MOP3;PASD3;Aryl hydrocarbon receptor nuclear translocator-like protein 1;Basic-helix-loop-helix-PAS protein MOP3Brain and muscle ARNT-like 1;Class E basic helix-loop-helix protein 5;bHLHe5;Member of PAS protein 3;PAS domain-containing protein 3;bHLH-PAS protein JAP3 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized Acetyl-peptide derived from human BMAL1 around the Acetylation site of Lys538. AA range:501-550 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® BMAL1 (Acetyl Lys538) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against BMAL1. Recognizes Acetyl. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | BMAL1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Acetyl-BMAL1 (K538) Polyclonal Antibody detects endogenous levels of BMAL1 protein only when acetylated at K538. |
| Molecular Weight | Observed: 70 kDa |
| UniProt / Swiss-Prot | O00327 (Human) | Q9WTL8 (Mouse) | Q9EPW1 (Rat) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized Acetyl-peptide derived from human BMAL1 around the Acetylation site of Lys538. AA range:501-550
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
