| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | SMARCA4;BAF190A;BRG1;SNF2B;SNF2L4;Transcription activator BRG1;ATP-dependent helicase SMARCA4;BRG1-associated factor 190A;BAF190A;Mitotic growth and transcription activator;Protein BRG-1;Protein brahma homolog 1;SNF2-beta;SWI/S |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Brg-1. AA range:1565-1614 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Brg-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against BRG1. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | BRG1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Brg-1 Polyclonal Antibody detects endogenous levels of Brg-1 protein. |
| Molecular Weight | Observed: 200 kDa |
| UniProt / Swiss-Prot | P51532 (Human) | Q3TKT4 (Mouse) | Q8K1P7 (Rat) |
Recommended dilutions: WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Brg-1. AA range:1565-1614
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
