| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ACTB;Actin;cytoplasmic 1;Beta-actin;Actin β;β-Actin;Beta actin;B actin;actin Beta |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from C. Elegans β-actin |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® C. Elegans β-actin Mouse Monoclonal Antibody (Mix-mA) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Actin β. It has been validated for Immunohistochemistry, Western Blot and reacts with C. elegans, Human, Mouse, Rat.
Validated Applications
| Target | Actin β |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | C. elegans, Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of C. Elegans β-actin |
| Molecular Weight | Observed: 42 kDa |
| UniProt / Swiss-Prot | P0DM41 (Human) |
Recommended dilutions: IHC1:200-400;WB 1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from C. Elegans β-actin
Reactivity Notes
Confirmed cross-reactivity: C. elegans, Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: IHC1:200-400;WB 1:5000-20000
Confirmed reactivity: C. elegans, Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
