| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | FOS;G0S7;Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
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| Conjugate | |
| Molecular weight | |
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Product Overview
U-Blot® c-Fos (Phospho Ser32) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against c-Fos. Recognizes Phospho (Ser32). It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | c-Fos |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1369R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | c-Fos (Phospho Ser32) Antibody detects endogenous levels of c-Fos protein only when phosphorylated at S32.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):YHsPA |
| Molecular Weight | Calculated: 41 kDa | Observed: 55 kDa |
| UniProt / Swiss-Prot | P01100 (Human) | P01101 (Mouse) | P12841 (Rat) |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Proto-oncogene c-Fos
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1369R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
