| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | RAF proto-oncogene serine/threonine-protein kinase;Proto-oncogene c-RAF;cRaf;Raf-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human C-RAF (Phospho Tyr340) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® C-RAF (Phospho Tyr340) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against c-Raf. Recognizes Phospho. It has been validated for Immunohistochemistry, Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | c-Raf |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of C-RAF (Phospho Tyr340) Rabbit pAb at Human, Mouse,Rat |
| Molecular Weight | Calculated: 71 kDa |
| UniProt / Swiss-Prot | P04049 (Human) | Q99N57 (Mouse) | P11345 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-200
Immunogen / Antigen
Synthesized peptide derived from human C-RAF (Phospho Tyr340)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: WB 1:500-2000;IHC 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
