U-Blot® Calpain 2 Rabbit Polyclonal Antibody

SKU:BHA21805867
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Calpain 2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WP11390-50 50 uL
WP11390-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Calpain 2
Antigen
  • Calpain-2 catalytic subunit
Alternative names CAPN2;CANPL2;Calpain-2 catalytic subunit;Calcium-activated neutral proteinase 2;CANP 2;Calpain M-type;Calpain large polypeptide L2;Calpain-2 large subunit;Millimolar-calpain;M-calpain
Gene ID 824 (Human) | 12334 (Mouse) | 29154 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human Calpain 2. AA range:305-354
Conjugate
  • Unconjugated
Molecular weight Calculated: 80 kDa
Purification Affinity Purified
Cellular localization Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP11390
Main SKU BHA21805867
Antibody

Product Overview

U-Blot® Calpain 2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Calpain 2. It has been validated for Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target Calpain 2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Calpain 2 Polyclonal Antibody detects endogenous levels of Calpain 2 protein.
Molecular Weight Calculated: 80 kDa
UniProt / Swiss-Prot P17655 (Human) | O08529 (Mouse) | Q07009 (Rat)

Recommended dilutions: IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human Calpain 2. AA range:305-354

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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