| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Caspase-2;CASP-2;Neural precursor cell expressed developmentally down-regulated protein 2;NEDD-2;Protease ICH-1;[Cleaved into: Caspase-2 subunit p18;Caspase-2 subunit p13;Caspase-2 subunit p12] |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Caspase 2 (p13, Cleaved-Gly334) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Caspase 2 (p13, Cleaved-Gly334) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Caspase-2. It has been validated for Western Blot, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Caspase-2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human Caspase 2 (p13, Cleaved-Gly334, protein was cleaved amino acid sequence between333-334 ) |
| Molecular Weight | Observed: 13 kDa,50 kDa |
| UniProt / Swiss-Prot | P42575 (Human) | P29594 (Mouse) | P55215 (Rat) |
Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Immunogen / Antigen
Synthesized peptide derived from human Caspase 2 (p13, Cleaved-Gly334)
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
alternative products:Isoforms differ in the N- and C-termini,catalytic activity:Strict requirement for an Asp residue at P1, with 316-asp being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-|-.,function:Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.,PTM:The mature protease can process its own propeptide, but not that of other caspases.,similarity:Belongs to the peptidase C14A family.,similarity:Contains 1 CARD domain.,subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Interacts with LRDD.,tissue specificity:Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle.
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
