| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Caspase-8;CASP-8;Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4;FADD-homologous ICE/ced-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MORT1-associated ced-3 homolog;MACH;[Cleaved into: Caspase-8 subunit p18;Caspase-8 subunit p10] |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Caspase 8 (Phospho Tyr448) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Caspase 8 (Phospho Tyr448) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Caspase-8. Recognizes Phospho. It has been validated for Immunohistochemistry, Western Blot and reacts with Human, Mouse.
Validated Applications
| Target | Caspase-8 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Caspase 8 (Phospho Tyr448) Rabbit pAb at Human, Mouse |
| Molecular Weight | Observed: 55 kDa |
| UniProt / Swiss-Prot | Q14790 (Human) | O89110 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-200
Immunogen / Antigen
Synthesized peptide derived from human Caspase 8 (Phospho Tyr448)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
caspase 8(CASP8) Homo sapiens This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alt
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: WB 1:500-2000;IHC 1:50-200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
