| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CD19;B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD antigen CD19 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD19 around the phosphorylation site of Tyr531. AA range:501-550 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CD19 (Phospho Tyr531) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CD19. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Monkey.
Validated Applications
| Target | CD19 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Monkey |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-CD19 (Y531) Polyclonal Antibody detects endogenous levels of CD19 protein only when phosphorylated at Y531. |
| Molecular Weight | Observed: 61 kDa |
| UniProt / Swiss-Prot | P15391 (Human) | P25918 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CD19 around the phosphorylation site of Tyr531. AA range:501-550
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Monkey. Confirm suitability for untested species before use.
Scientific Background
Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.