| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CD28;T-cell-specific surface glycoprotein CD28;TP44;CD antigen CD28 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CD28 (Phospho Tyr218) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CD28. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human.
Validated Applications
| Target | CD28 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218. |
| Molecular Weight | Observed: 45 kDa |
| UniProt / Swiss-Prot | P10747 (Human) | P31041 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CD28 around the phosphorylation site of Tyr218. AA range:171-220
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
