| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CD3E;T3E;T-cell surface glycoprotein CD3 epsilon chain;T-cell surface antigen T3/Leu-4 epsilon chain;CD antigen CD3e |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD3-epsilon. AA range:22-71 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CD3-ε Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CD3E. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse, Monkey.
Validated Applications
| Target | CD3E |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | CD3-ε Polyclonal Antibody detects endogenous levels of CD3-ε protein. |
| Molecular Weight | Observed: 21 kDa |
| UniProt / Swiss-Prot | P07766 (Human) | P22646 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CD3-epsilon. AA range:22-71
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse, Monkey. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
