| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ICAM3;Intercellular adhesion molecule 3;ICAM-3;CDw50;ICAM-R;CD antigen CD50 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD50/ICAM-3 around the phosphorylation site of Ser518. AA range:484-533 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CD50 (Phospho Ser518) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CD50/ICAM-3. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human.
Validated Applications
| Target | CD50/ICAM-3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-CD50 (S518) Polyclonal Antibody detects endogenous levels of CD50 protein only when phosphorylated at S518. |
| Molecular Weight | Observed: 42-60 kDa |
| UniProt / Swiss-Prot | P32942 (Human) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CD50/ICAM-3 around the phosphorylation site of Ser518. AA range:484-533
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
