| Field | Specification |
|---|---|
| Alternative Names | T-lymphocyte activation antigen CD86;Activation B7-2 antigen;B70;BU63;CTLA-4 counter-receptor B7.2;FUN-1;CD antigen CD86 |
| Antigen | |
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| Clonality | |
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| Formulation | |
| Gene ID | |
| Host | |
| Isotype | |
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| Target |
Product Overview
U-Blot® CD86 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against CD86. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Mouse.
Validated Applications
| Target | CD86 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0048R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 60-80 kDa |
| UniProt / Swiss-Prot | P42081 (Human) | P42082 (Mouse) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-5000;IF 1:200-1000;ELISA 1:5000-20000
Immunogen / Antigen
CD86
Reactivity Notes
Confirmed cross-reactivity: Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-5000;IF 1:200-1000;ELISA 1:5000-20000
Confirmed reactivity: Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0048R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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