| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CDK1;CDC2;CDC28A;CDKN1;P34CDC2;Cyclin-dependent kinase 1;CDK1;Cell division control protein 2 homolog;Cell division protein kinase 1;p34 protein kinase |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDC2 around the phosphorylation site of Tyr15. AA range:5-54 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CDC2 (Phospho Tyr15) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CDK1/CDC2. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | CDK1/CDC2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Cdc2 (Y15) Polyclonal Antibody detects endogenous levels of Cdc2 protein only when phosphorylated at Y15. |
| Molecular Weight | Observed: 34 kDa |
| UniProt / Swiss-Prot | P06493 (Human) | P11440 (Mouse) | P39951 (Rat) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CDC2 around the phosphorylation site of Tyr15. AA range:5-54
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
