| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CDCA3;C8;GRCC8;TOME1;Cell division cycle-associated protein 3;Gene-rich cluster protein C8;Trigger of mitotic entry protein 1;TOME-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDCA3. AA range:219-268 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CdcA3 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CdcA3. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | CdcA3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | CdcA3 Polyclonal Antibody detects endogenous levels of CdcA3 protein. |
| Molecular Weight | Observed: 28 kDa |
| UniProt / Swiss-Prot | Q99618 (Human) | Q99M54 (Mouse) | Q68FW2 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CDCA3. AA range:219-268
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
domain:The KEN box is required for the association with the APC/C-Cdh1 complex.,function:F-box-like protein which is required for entry into mitosis. Acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Ubiquitinated and degraded by the APC/C-Cdh1 complex.,subunit:Interacts with SKP1A. Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:5000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
