| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cdk 4;cdk4;CDK4 protein;CDK4_HUMAN;Cell division kinase 4;Cell division protein kinase 4;CMM 3;CMM3;Crk3;Cyclin dependent kinase 4;Cyclin-dependent kinase 4;Melanoma cutaneous malignant 3;MGC14458;p34 cdk4;PSK J3;PSK-J3. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human CDK4 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CDK4 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CDK4. It has been validated for Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | CDK4 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of CDK4 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 33 kDa |
| UniProt / Swiss-Prot | P11802 (Human) | P30285 (Mouse) |
Recommended dilutions: WB 1:1000
Immunogen / Antigen
Purified recombinant human CDK4 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
cyclin dependent kinase 4(CDK4) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
