U-Blot® Cdk9 Mouse Monoclonal Antibody

SKU:BHA21801691
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Cdk9 antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human.
Clonality Monoclonal
Host Mouse
Reactivity Human
Conjugate Unconjugated
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WM0112-50 50 uL
WM0112-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Cdk9
Antigen
  • Cell division protein kinase 9
Alternative names CDK9;CDC2L4;TAK;Cyclin-dependent kinase 9;C-2K;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit
Gene ID 1025 (Human)
Host Mouse
Clonality
  • Monoclonal
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Immunogen Purified recombinant fragment of human Cdk9 expressed in E. Coli.
Conjugate
  • Unconjugated
Molecular weight Calculated: 43 kDa
Purification Affinity Purified
Cellular localization Nucleus. Cytoplasm. Nucleus, PML body. Accumulates on chromatin in response to replication stress. Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated.
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM0112
Main SKU BHA21801691
Antibody

Product Overview

U-Blot® Cdk9 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cdk9. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.

Validated Applications

Target Cdk9
Host Mouse
Clonality Monoclonal
Conjugate Unconjugated
Reactivity Human
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Affinity Purified
Specificity Cdk9 Monoclonal Antibody detects endogenous levels of Cdk9 protein.
Molecular Weight Calculated: 43 kDa
UniProt / Swiss-Prot P50750 (Human) | Q99J95 (Mouse)

Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200

Immunogen / Antigen

Purified recombinant fragment of human Cdk9 expressed in E. Coli.

Reactivity Notes

Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.

Scientific Background

cyclin dependent kinase 9(CDK9) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:200-1:1000;ELISA 1:10000;IF 1:50-200

What species does it react with?

Confirmed reactivity: Human. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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