| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CDK9;CDC2L4;TAK;Cyclin-dependent kinase 9;C-2K;Cell division cycle 2-like protein kinase 4;Cell division protein kinase 9;Serine/threonine-protein kinase PITALRE;Tat-associated kinase complex catalytic subunit |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186. AA range:152-201 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Cdk9 (Phospho Thr186) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Cdk9. Recognizes Phospho. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cdk9 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Cdk9 (T186) Polyclonal Antibody detects endogenous levels of Cdk9 protein only when phosphorylated at T186. |
| Molecular Weight | Observed: 42 kDa |
| UniProt / Swiss-Prot | P50750 (Human) | Q99J95 (Mouse) | Q641Z4 (Rat) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CDK9 around the phosphorylation site of Thr186. AA range:152-201
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
cyclin dependent kinase 9(CDK9) Homo sapiens The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:5000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
