| Field | Specification |
|---|---|
| Target | |
| Alternative names | 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha;Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase;Snf2-like;. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CHD3(C-term) Mouse Monoclonal Antibody (WM0586) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CHD3. It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation and reacts with Human, Mouse, Rat.
Validated Applications
| Target | CHD3 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins. |
| Molecular Weight | Observed: 260 kDa |
| UniProt / Swiss-Prot | Q12873 (Human) |
Recommended dilutions: WB 1:1000;ICC 1:400
Immunogen / Antigen
Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation. Recommended dilutions: WB 1:1000;ICC 1:400
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
