| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CASP1;IL1BC;IL1BCE;Caspase-1;CASP-1;Interleukin-1 beta convertase;IL-1BC;Interleukin-1 beta-converting enzyme;ICE;IL-1 beta-converting enzyme;p45;[Cleaved into: Caspase-1 subunit p20;Caspase-1 subunit p10] |
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| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
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Product Overview
U-Blot® Cleaved Caspase-1 (Asp296) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Caspase-1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Mouse.
Validated Applications
| Target | Caspase-1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1527R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 45 kDa | Observed: 22 kDa |
| UniProt / Swiss-Prot | P29466 (Human) | P29452 (Mouse) | P43527 (Rat) |
Recommended dilutions: IHC 1:100-1:200;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Caspase-1
Reactivity Notes
Confirmed cross-reactivity: Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:200;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1527R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
