| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CASP3;CPP32;Caspase-3;CASP-3;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:157-206 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Cleaved-Caspase-3 p12 (D175) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Caspase-3. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Caspase-3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Cleaved-Caspase-3 p12 (D175) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-3 p12 protein resulting from cleavage adjacent to D175. |
| Molecular Weight | Observed: 12 kDa,35 kDa |
| UniProt / Swiss-Prot | P42574 (Human) | P70677 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;IF 1:50-300
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:157-206
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;IF 1:50-300
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
