| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Collagen alpha-1;II;chain;Alpha-1 type II collagen;[Cleaved into: Collagen alpha-1;II;chain;Chondrocalcin] |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Collagen II |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Collagen II Mouse Monoclonal Antibody (7F9) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Collagen II. It has been validated for Immunohistochemistry, Immunofluorescence and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Collagen II |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7F9 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Collagen II protein |
| Molecular Weight | Observed: 142 kDa |
| UniProt / Swiss-Prot | P02458 (Human) | P28481 (Mouse) | P05539 (Rat) |
Recommended dilutions: IHC 1:50-300;IF 1:50-200
Immunogen / Antigen
Synthetic Peptide of Collagen II
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence. Recommended dilutions: IHC 1:50-300;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 7F9.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
