| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Collagen alpha-1;IV;chain [Cleaved into: Arresten] |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Collagen Type IV AA range: 1600-1669 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Collagen IV Mouse Monoclonal Antibody (8E5) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Collagen IV. It has been validated for Immunofluorescence, Immunohistochemistry and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Collagen IV |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 8E5 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Collagen IV protein |
| Molecular Weight | Observed: 161 kDa |
| UniProt / Swiss-Prot | p02462 (Human) | P02463 (Mouse) |
Recommended dilutions: IF 1:50-200;IHC 1:50-300
Immunogen / Antigen
Synthesized peptide derived from human Collagen Type IV AA range: 1600-1669
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Safety & Handling
This antibody is validated for Immunofluorescence, Immunohistochemistry. Recommended dilutions: IF 1:50-200;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 8E5.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
