| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | COX4I2;COX4L2;Cytochrome c oxidase subunit 4 isoform 2;mitochondrial;Cytochrome c oxidase subunit IV isoform 2;COX IV-2 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human COX4I2 (C-terminus) protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® COX4I2 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against COX4I2. It has been validated for Western Blot, Flow Cytometry, Immunofluorescence and reacts with Human.
Validated Applications
| Target | COX4I2 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Flow Cytometry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | COX4I2 Monoclonal Antibody detects endogenous levels of COX4I2 protein. |
| Molecular Weight | Calculated: 20 kDa |
| UniProt / Swiss-Prot | Q96KJ9 (Human) | Q91W29 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;Flow Cyt 1:100-1:200;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human COX4I2 (C-terminus) protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Flow Cytometry, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;Flow Cyt 1:100-1:200;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
