| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CREB1;Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CREB-1 (Phospho Ser133) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against CREB-1. Recognizes Phospho. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat, Pig.
Validated Applications
| Target | CREB-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Pig |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133. |
| Molecular Weight | Observed: 37 kDa |
| UniProt / Swiss-Prot | P16220 (Human) | Q01147 (Mouse) | P15337 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;ELISA 1:10000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Pig. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;ELISA 1:10000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat, Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
