| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CSK;Tyrosine-protein kinase CSK;C-Src kinase;Protein-tyrosine kinase CYL |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant fragment of human Csk expressed in E. Coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® CSK Mouse Monoclonal Antibody (WM0127) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against CSK. It has been validated for Western Blot, Immunofluorescence, Flow Cytometry, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | CSK |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunofluorescence, Flow Cytometry, ELISA |
| Purification | Affinity Purified |
| Specificity | Csk Monoclonal Antibody detects endogenous levels of Csk protein. |
| Molecular Weight | Calculated: 51 kDa |
| UniProt / Swiss-Prot | P41240 (Human) | P41241 (Mouse) | P32577 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant fragment of human Csk expressed in E. Coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.,PTM:Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,subcellular location:Mainly cytoplasmic, also present in lipid rafts.,subunit:Interacts with PTPN8 (By similarity). Interacts with phosphorylated SIT1, PAG1, LIME1 and TGFB1I1.,tissue specificity:Expressed in lung and macrophages.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Flow Cytometry, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;Flow Cyt 1:200-1:400;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
