| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CCNB1;CCNB;G2/mitotic-specific cyclin-B1 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Cyclin B1 (Phospho Ser126) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Cyclin B1. Recognizes Phospho (Ser126). It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human.
Validated Applications
| Target | Cyclin B1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0070R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | This antibody detects endogenous levels of Cyclin B1 only when phosphorylated at Ser126.This antibody does not recognize phosphorylated at other sites. |
| Molecular Weight | Calculated: 48 kDa | Observed: 55 kDa |
| UniProt / Swiss-Prot | P14635 (Human) | P24860 (Mouse) | P30277 (Rat) |
Recommended dilutions: IHC 1:500-1:2000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
CCNB1
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:500-1:2000;WB 1:1000-1:5000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0070R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
