| Field | Specification |
|---|---|
| Alternative Names | CCNE2;G1/S-specific cyclin-E2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Cyclin E2 around the phosphorylation site of Thr392. AA range:355-404 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Cyclin E2 (Phospho Thr392) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Cyclin E2. Recognizes Phospho. It has been validated for Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | Cyclin E2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Cyclin E2 (T392) Polyclonal Antibody detects endogenous levels of Cyclin E2 protein only when phosphorylated at T392. |
| Molecular Weight | Calculated: 47 kDa |
| UniProt / Swiss-Prot | O96020 (Human) | Q9Z238 (Mouse) |
Recommended dilutions: IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Cyclin E2 around the phosphorylation site of Thr392. AA range:355-404
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2. This cyclin has been shown to specifically interact with CIP/KIP family of CDK inhibitors, and plays a role in cell cycle G1/S transition. The expression of this gene peaks at the G1-S phase and exhibits a pattern of tissue specificity distinct from that of cyclin E1. A significantly increased expression level of this gene was observed in tumor-derived cells. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Immunofluorescence, ELISA. Recommended dilutions: IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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