| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CARD2;CK 8;CK-8;CK8;CYK8;CYKER;Cytokeratin endo A;Cytokeratin-8;DreK8;EndoA;K2C8;K2C8_HUMAN;K8;Keratin 8;Keratin type II cytoskeletal 8;Keratin, type II cytoskeletal 8;Keratin-8;KO;Krt 2.8;KRT8;MGC118110;MGC174782;MGC53564;MGC85764;sb:cb186;Type-II keratin Kb8 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human Cytokeratin 8 AA range: 400-483 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Cytokeratin 8 (ABT535) Mouse Monoclonal Antibody (WM1557) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Cytokeratin 8. It has been validated for Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Cytokeratin 8 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT535 |
| Isotype | IgG2a, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody can specifically recognize human CK8 protein, and shows no cross reaction with CK1, 4, 5, 6, 7, 10, 15, 16, 17, 18, 20. |
| Molecular Weight | Calculated: 54 kDa | Observed: 54 kDa |
| UniProt / Swiss-Prot | P05787 (Human) | P11679 (Mouse) | Q10758 (Rat) |
Recommended dilutions: IHC 1:200-400;ELISA 1:500-5000
Immunogen / Antigen
Synthesized peptide derived from human Cytokeratin 8 AA range: 400-483
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Safety & Handling
This antibody is validated for Immunohistochemistry, ELISA. Recommended dilutions: IHC 1:200-400;ELISA 1:500-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT535.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
