| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DBF4;ASK;DBF4A;ZDBF1;Protein DBF4 homolog A;Activator of S phase kinase;Chiffon homolog A;DBF4-type zinc finger-containing protein 1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Dbf4 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Dbf4. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Monkey.
Validated Applications
| Target | Dbf4 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Dbf4 Polyclonal Antibody detects endogenous levels of Dbf4 protein. |
| Molecular Weight | Observed: 77 kDa |
| UniProt / Swiss-Prot | Q9UBU7 (Human) | Q9QZ41 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human DBF4. AA range:10-59
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Monkey. Confirm suitability for untested species before use.
Scientific Background
function:Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.,induction:Induced in G1 phase at low level, increased during G1-S phase and remain high during S and G2-M phase.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 DBF4-type zinc finger.,similarity:Contains 2 BRCT domains.,subunit:Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4A or DBF4B. Such complexes are stable upon replication stress. Interacts with MEN1, MCM2, ORC2L, ORC4L and ORC6L.,tissue specificity:Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
