| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Flag-Tag |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® DDDDK-Tag(binds to flag sequence) Mouse Monoclonal Antibody (12M11) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Flag-tag. It has been validated for Western Blot, ELISA, Immunoprecipitation, Immunofluorescence and reacts with the listed species.
Validated Applications
| Target | Flag-tag |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12M11 |
| Isotype | IgG1, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Applications | Western Blot, ELISA, Immunoprecipitation, Immunofluorescence |
| Purification | Protein A |
| Specificity | This recombinant antibody can highly specifically recognize the Flag tags of C-terminal and N-terminal of recombinant protein. Variable region gene of this antibody comes from the mouse gene library immunized with DDDK peptide. Constant region of this antibody is mouse IgG1 and mouse kappa. |
Recommended dilutions: WB 1:10000-50000;ELISA 1:20000-50000;IP 1:50-1:200;IF 1:1000 -3000
Immunogen / Antigen
Synthetic Peptide of Flag-Tag
Scientific Background
The DYKDDDDK peptide (Flag-tag) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, and then used to separate recombinant, over expressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.; Mouse VH IgG1 and VL Kappa was expressed from 293F cells
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunoprecipitation, Immunofluorescence. Recommended dilutions: WB 1:10000-50000;ELISA 1:20000-50000;IP 1:50-1:200;IF 1:1000 -3000
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 12M11.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
