| Field | Specification |
|---|---|
| Target | |
| Alternative names | DDDDK epitope tag;DDDDK epitope tag;DYKDDDDK epitope tag |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | DDDDK synthetic peptide conjugated to KLH. |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® DDDDK-Tag(binds to flag sequence) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against FLAG-tag. It has been validated for Western Blot, ELISA, Immunoprecipitation, Immunofluorescence and reacts with the listed species.
Validated Applications
| Target | FLAG-tag |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Applications | Western Blot, ELISA, Immunoprecipitation, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells. |
Recommended dilutions: WB 1:1000-1:3000;ELISA 1:5000-20000;IF 1:50-200
Immunogen / Antigen
DDDDK synthetic peptide conjugated to KLH.
Scientific Background
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunoprecipitation, Immunofluorescence. Recommended dilutions: WB 1:1000-1:3000;ELISA 1:5000-20000;IF 1:50-200
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
