U-Blot® DDX1 Rabbit Polyclonal Antibody

SKU:BHA21811283
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    Overview
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    Anti-DDX1 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot with reactivity to Human, Mouse, Rat.
    Clonality Polyclonal
    Host Rabbit
    Reactivity Human, Mouse, Rat
    Conjugate Unconjugated
    Isotype IgG
    Applications Western Blot
    Options selector
    Catalog no. Size
    WP16313-50 50 uL
    WP16313-100 100 uL
    Available Options

    Select the variant that best fits your experiment. Availability and lead time may vary by option.

    • Options: Size: 50 uL
    • Lead time: options listed in "Availability Content"; other statuses may take longer.
    • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
    • Shipping: cold-chain shipment (typically with ice packs).
    • Upon receipt: store at recommended temperature as soon as possible.
    • Sales terms and conditions: Please review prior to ordering.
    Field Specification
    Antigen
    • DDX1
    Cellular Localization Nucleus. Cytoplasm. Cytoplasmic granule. Cytoplasm, cytosol . Mitochondrion . Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (PubMed:24608264). Colocalizes in the cytosol with DDX21, DHX36 and TICAM1. Colocalizes in the mitochondria with TICAM1 and poly(I:C) RNA ligand. The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) stimulation (By similarity). .; Cytoplasm . (Microbial infection) Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells (PubMed:20573827). Required for proper localization of HIV-1 Rev (PubMed:15567440). .
    Clonality
    • Polyclonal
    Concentration 1 mg/ml
    Conjugate
    • Unconjugated
    Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
    Gene ID 1653 (Human) | 104721 (Mouse) | 84474 (Rat)
    Host Rabbit
    Immunogen Synthesized peptide derived from human DDX1 AA range: 559-609
    Isotype
    • IgG
    Molecular Weight Calculated: 81 kDa
    Product Type
    • Polyclonal Antibody
    • Primary Antibodies
    Reactivity
    • Human
    • Mouse
    • Rat
    Storage -15°C to -25°C/1 year(Do not lower than -25°C)
    Target DDX1
    Antibody

    Product Overview

    U-Blot® DDX1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against DDX1. It has been validated for Western Blot and reacts with Human, Mouse, Rat.

    Validated Applications

    Target DDX1
    Host Rabbit
    Clonality Polyclonal
    Isotype IgG
    Conjugate Unconjugated
    Reactivity Human, Mouse, Rat
    Applications Western Blot
    Purification Affinity Purified
    Specificity This antibody detects endogenous levels of DDX1 at Human/Mouse/Rat
    Molecular Weight Calculated: 81 kDa
    UniProt / Swiss-Prot Q92499 (Human) | Q91VR5 (Mouse) | Q641Y8 (Rat)

    Recommended dilutions: WB 1:500-2000

    Immunogen / Antigen

    Synthesized peptide derived from human DDX1 AA range: 559-609

    Reactivity Notes

    Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

    Scientific Background

    DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. [provided by RefSeq, Jul 2008]

    Safety & Handling

    For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
    What applications is this antibody validated for?

    This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-2000

    What species does it react with?

    Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

    Is this antibody conjugated?

    Conjugate: Unconjugated.

    How should it be stored?

    Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

    Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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