U-Blot® DNA pol δ Rabbit Polyclonal Antibody

SKU:BHA21806575
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-DNA pol δ cat antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP12033-50 50 uL
WP12033-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target DNA pol δ cat
Antigen
  • DNA polymerase delta catalytic subunit
Alternative names POLD1;POLD;DNA polymerase delta catalytic subunit;DNA polymerase subunit delta p125
Gene ID 5424 (Human) | 18971 (Mouse) | 59294 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
Immunogen The antiserum was produced against synthesized peptide derived from human POLD1. AA range:1051-1100
Conjugate
  • Unconjugated
Molecular weight Observed: 110, 124 kDa
Purification Affinity Purified
Cellular localization Nucleus . Colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). After UV irradiation, recruited to DNA damage sites within 2 hours, independently on the cell cycle phase, nor on PCNA ubiquitination. This recruitment requires POLD3, PCNA and RFC1-replication factor C complex (PubMed:20227374, PubMed:22801543). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP12033
Main SKU BHA21806575
Antibody

Product Overview

U-Blot® DNA pol δ Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against DNA pol δ cat. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target DNA pol δ cat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity DNA pol δ cat Polyclonal Antibody detects endogenous levels of DNA pol δ cat protein.
Molecular Weight Observed: 110 124 kDa
UniProt / Swiss-Prot P28340 (Human) | P52431 (Mouse) | O54747 (Rat)

Recommended dilutions: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.

Immunogen / Antigen

The antiserum was produced against synthesized peptide derived from human POLD1. AA range:1051-1100

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:40000;Not yet tested in other applications.

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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