| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | DCTN1;Dynactin subunit 1;150 kDa dynein-associated polypeptide;DAP-150;DP-150;p135;p150-glued |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human Dynactin 1 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Dynactin 1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Dynactin 1. It has been validated for Western Blot, Immunofluorescence and reacts with Human, Mouse, Bovine, Pig.
Validated Applications
| Target | Dynactin 1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Bovine, Pig |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | Dynactin 1 Monoclonal Antibody detects endogenous levels of Dynactin 1 protein. |
| Molecular Weight | Calculated: 142 kDa |
| UniProt / Swiss-Prot | Q14203 (Human) | O08788 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human Dynactin 1 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Bovine, Pig. Confirm suitability for untested species before use.
Scientific Background
This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Bovine, Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
