| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human E-Cadherin AA range: 700-800 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® E-cadherin (ABT270) Mouse Monoclonal Antibody (WM1583) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against E-cadherin. It has been validated for Immunohistochemistry, ELISA and reacts with Human.
Validated Applications
| Target | E-cadherin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT270 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody can specifically recognize E-cadherin protein. |
| Molecular Weight | Calculated: 97 kDa | Observed: 120 kDa |
| UniProt / Swiss-Prot | P12830 (Human) |
Recommended dilutions: IHC 1:200-400;ELISA 1:500-5000
Immunogen / Antigen
Synthesized peptide derived from human E-Cadherin AA range: 700-800
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]
Safety & Handling
This antibody is validated for Immunohistochemistry, ELISA. Recommended dilutions: IHC 1:200-400;ELISA 1:500-5000
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT270.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
