| Field | Specification |
|---|---|
| Target | |
| Alternative names | Dmel\CG6376;Dmel_CG6376;drosE2F1;E;Sev-CycE;3A;E;var;3-93E;E2-promoter binding facto;E2F 1;E2F transcription factor 1;E2F-1;E2f-PA;E2f-PB;E2f-PC;E2F1;E2f1 E2F transcription factor 1;E2F1_HUMAN;Evar;3;164;KIAA4009;l;3;07172;l;3;j3B1;l;3;j3C2;l;3;rM729;mKIAA4009;OTTHUMP00000030661;PBR3;PRB binding protein E2F 1;PRB-binding protein E2F-1;RBAP 1;RBAP-1;RBAP1;RBBP-3;RBBP3;RBP 3;RBP3;Retinoblastoma-associated protein 1;Retinoblastoma-binding protein 3;Transcription factor E2F1. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human E2F-1 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® E2F-1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against E2F-1. It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation and reacts with Human, Rat.
Validated Applications
| Target | E2F-1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of E2F-1 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | Q01094 (Human) | Q61501 (Mouse) |
Recommended dilutions: WB 1:500;ICC 1:100;IF 1:50-200
Immunogen / Antigen
Purified recombinant human E2F-1 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation. Recommended dilutions: WB 1:500;ICC 1:100;IF 1:50-200
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
