| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | E2F1 RBBP3 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthetic peptide from human protein at AA range: 100-170 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® E2F-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against E2F-1. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | E2F-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous E2F-1 protein |
| Molecular Weight | Observed: 60 kDa |
| UniProt / Swiss-Prot | Q01094 (Human) | Q61501 (Mouse) | O09139 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300
Immunogen / Antigen
Synthetic peptide from human protein at AA range: 100-170
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-2000;ELISA 1:10000-20000;IHC 1:50-300
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
