| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | EEA1;ZFYVE2;Early endosome antigen 1;Endosome-associated protein p162;Zinc finger FYVE domain-containing protein 2 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® EEA1 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against EEA1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | EEA1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0188R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 162 kDa | Observed: 162 kDa |
| UniProt / Swiss-Prot | Q15075 (Human) | Q8BL66 (Mouse) |
Recommended dilutions: IHC 1:100-200;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000
Immunogen / Antigen
Early endosome antigen 1
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
disease:Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.,domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.,function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 FYVE-type zinc finger.,subunit:Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-200;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0188R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
