| Field | Specification |
|---|---|
| Alternative Names | EEA1;ZFYVE2;Early endosome antigen 1;Endosome-associated protein p162;Zinc finger FYVE domain-containing protein 2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EEA1. AA range:205-254 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® EEA1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against EEA1. It has been validated for Western Blot, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | EEA1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | EEA1 Polyclonal Antibody detects endogenous levels of EEA1 protein. |
| Molecular Weight | Observed: 162 kDa |
| UniProt / Swiss-Prot | Q15075 (Human) | Q8BL66 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human EEA1. AA range:205-254
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
disease:Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.,domain:The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.,function:Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 FYVE-type zinc finger.,subunit:Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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