U-Blot® EF-1 α1/2 (Acetyl Lys146) Rabbit Polyclonal Antibody

SKU:BHA21803717
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-EF-1 α1/2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Mouse, Rat.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP0378-50 50 uL
WP0378-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target EF-1 α1/2
Antigen
  • EF-1 α1/2 (Acetyl Lys146)
Alternative names Elongation factor 1-alpha 1;EF-1-alpha-1;Elongation factor Tu;EF-Tu;Eukaryotic elongation factor 1 A-1;eEF1A-1;Leukocyte receptor cluster member 7
Gene ID 1915 (Human) | 13627 (Mouse) | 171361 (Rat)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • ELISA
Immunogen Synthesized peptide derived from human EF-1 α1/2 (Acetyl Lys146)
Conjugate
  • Unconjugated
Molecular weight Observed: 51 kDa
Purification Affinity Purified
Cellular localization Cytoplasm . Nucleus . Nucleus, nucleolus . Cell membrane . Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934). .
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP0378
Main SKU BHA21803717
Antibody

Product Overview

U-Blot® EF-1 α1/2 (Acetyl Lys146) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against EF-1 α1/2. Recognizes Acetyl. It has been validated for Western Blot, ELISA and reacts with Human, Mouse, Rat.

Validated Applications

Target EF-1 α1/2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity This antibody detects endogenous levels of Human,Mouse,Rat EF-1 α1/2 (Acetyl Lys146)
Molecular Weight Observed: 51 kDa
UniProt / Swiss-Prot P68104;Q05639;Q5VTE0 (Human) | P10126 (Mouse) | P62630 (Rat)

Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000

Immunogen / Antigen

Synthesized peptide derived from human EF-1 α1/2 (Acetyl Lys146)

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.

Scientific Background

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:1000-2000;ELISA 1:5000-20000

What species does it react with?

Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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