| Field | Specification |
|---|---|
| Alternative Names | EGFR;ERBB;ERBB1;HER1;Epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr678. AA range:651-700 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® EGFR (Phospho Thr678) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against EGFR. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | EGFR |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-EGFR (T678) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at T678. |
| Molecular Weight | Observed: 175 kDa |
| UniProt / Swiss-Prot | P00533 (Human) | Q01279 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr678. AA range:651-700
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
Research budgets are tight — we get it. That's why we've put together a fresh round of exclusive promotions designed to help you stock up on the reagents, kits, and consumables your lab depends on, without stretching your budget.
🔬 What's on offer right now:
10% Off Pre-Designed siRNA Sets
20% Off Transmembrane Proteins
50% Off Lab Consumables + Free Shipping
$99 Pipette Filler Promotion Package
BlasTaq 2X qPCR MasterMix - 50% OFF Limited Time Offer
DENARASE® Endonuclease — 10% Off One Order
