| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | EGFR;ERBB;ERBB1;HER1;Epidermal growth factor receptor;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1 |
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| Isotype | |
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Product Overview
U-Blot® EGFR (Phospho Tyr1068) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against EGFR. Recognizes Phospho (Tyr1068). It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | EGFR |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0895R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Phospho-EGFR (Y1068) Antibody detects endogenous levels of EGFR protein only when phosphorylated at Y1092.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):LQrYS |
| Molecular Weight | Calculated: 134 kDa | Observed: 175 kDa |
| UniProt / Swiss-Prot | P00533 (Human) | Q01279 (Mouse) |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
EGF Receptor (Tyr1068)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0895R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
