| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | EIF3F;EIF3S5;Eukaryotic translation initiation factor 3 subunit F;eIF3f;Deubiquitinating enzyme eIF3f;Eukaryotic translation initiation factor 3 subunit 5;eIF-3-epsilon;eIF3 p47 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® eIF3ε Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against eIF3ε. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | eIF3ε |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | eIF3ε Polyclonal Antibody detects endogenous levels of eIF3ε protein. |
| Molecular Weight | Observed: 38 kDa |
| UniProt / Swiss-Prot | O00303 (Human) | Q9DCH4 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human EIF3F. AA range:81-130
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,similarity:Belongs to the eIF-3 subunit F family.,similarity:Contains 1 MPN (JAB/Mov34) domain.,subunit:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
