| Field | Specification |
|---|---|
| Alternative Names | ITK;EMT;LYK;Tyrosine-protein kinase ITK/TSK;Interleukin-2-inducible T-cell kinase;IL-2-inducible T-cell kinase;Kinase EMT;T-cell-specific kinase;Tyrosine-protein kinase Lyk |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Emt (Phospho Tyr512) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Emt. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | Emt |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Emt (Y512) Polyclonal Antibody detects endogenous levels of Emt protein only when phosphorylated at Y512. |
| Molecular Weight | Observed: 72 kDa |
| UniProt / Swiss-Prot | Q08881 (Human) | Q03526 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human ITK around the phosphorylation site of Tyr512. AA range:478-527
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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